Cyto-gcamp6f
WebDec 16, 2024 · Cyto-GCaMP6f fluorescence is localized to Müller glia and a subset of neurons. Scale bar: 10 µm. Right, average projection of IPL in Slc1a3-CreER;Lck-GCaMP6f flox retinas at P10 following incubation in vehicle or 200 µM BAPTA-AM. Lck-GCaMP6f fluorescence is localized to Müller glial processes. GCaMP is a genetically encoded calcium indicator (GECI) initially developed in 2001 by Junichi Nakai. It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light-chain kinase. When bound to Ca , GCaMP fluoresces green with a peak excitation wavelength of 480 nm and a peak emission wavelength of 510 nm. It is used in biological …
Cyto-gcamp6f
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WebThese results clearly demonstrated that the GCaMP6f-mediated Ca 2+ signal from either neurons or astrocytes is specifically detectable in vivo via optical fiber, showing distinct temporal features to sensory stimulation. WebLck-GCaMP6f revealed entire bushy astrocytes, whereas cyto-GCaMP6f revealed the somata, major branches and branchlets (Supp Figure 3 A,B; n = 4 mice). Moreover, with live slice imaging we could...
WebLck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions … WebDec 3, 2024 · Consistent with the high efficiency and specificity of GCaMP6f labeling in our previous studies (Qin et al., 2024; Zhang et al., 2024), the microinjection of this virus resulted in reliable and...
WebDec 16, 2024 · Cyto-GCaMP6f fluorescence is localized to Müller glia and a subset of neurons. Scale bar: 10 µm. Right, average projection of IPL in Slc1a3-CreER;Lck-GCaMP6f flox retinas at P10 following incubation in vehicle or 200 µM BAPTA-AM. Lck-GCaMP6f fluorescence is localized to Müller glial processes. WebDec 21, 2016 · Cyto-GCaMP6f and Lck-GCaMP6f expression was sparse with Slc1a3-Cre/ERT mice, but expression of both GECIs was ubiquitous and high with Aldh1l1 …
WebSep 30, 2024 · Two 300 nl injections (600 nl total virus) of AAV5-GFaABC1D.cyto-GCaMP6f were made into visual cortex (0.5–1.0 mm anterior and 1.75–2.5 mm lateral of bregma) at a depth of 0.2–0.3 mm and 0.5 ...
WebDec 16, 2024 · CRITICAL: For calcium imaging experiments, a mix of both viral constructions (AAV2.5-gfaABC1D-cyto-GCaMP6f (viral titer range 2–6 × 10 13 vg/ml) and AVV2.5-GFAP-Cre-mCherry (4.3 × 10 12 vg/ml; 1:1 ratio) are injected. c. Place the tip of the needle in the first position and make a mark scratching softly on the surface of the skull … pic of a flying machineWebDec 3, 2024 · The fluorescent genetically encoded Ca 2+ indicator (GCaMP6f) was excited by light (wavelength of 488 nm) delivered by a laser (OBIS 488 LX-50 mW, Coherent, Santa Clara, CA, USA), and the beam intensity of the laser was accurately adjusted by the current. pic of a football to printpic of a german shepherd dogWebTwo 300nL injections (600nL total virus) of AAV5-GFaABC1D.cyto-GCaMP6f were made into visual cortex (0.5–1.0mm anterior and 1.75–2.5mm lateral of bregma) at a depth of 0.2–0.3mm and 0.5mm from the pial surface, respectively. Virus was injected at a rate of 2nL/s, with a 10min wait following each injection to allow for diffusion. pic of a goldendoodleWebNov 10, 2024 · Astrocytes show visual responses at their somata. (A) Average images of cyto-GCaMP6f-expressing astrocytes. Regions colored in blue indicate the individual … pic of africa flagWebAt left are shown the protein subunits of the integral membrane cytochrome b 6 f complex of the cyanobacterium, Mastigocladus laminosus (Kurisu, et al., 2003), oriented with the … pic of a great daneWebLck-GCaMP6f is a detector of single neuronal action potentials with fast response kinetics. It has an Lck-tag to improve its ability to reveal calcium signals in near membrane regions and fine processes ( e.g. , astrocyte processes). After Cre exposure, bright GCaMP6f fluorescence is observed following calcium binding (such as neuronal ... top beach resorts in new jersey