Bjhtert cell line
WebA breakthrough in cell biology research. Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology research … WebMar 14, 2016 · BJ-hTert, which are human normal foreskin fibroblasts immortalized with hTERT, 48 were purchased from the ATCC (#CRL-4001). 1BR3G human skin fibroblasts …
Bjhtert cell line
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WebOnly BJhTERT cells, with intact mitochondrial respiratory chain function were affected by light insult. Finally, we show that exposure of anaesthetized pigmented rat eye to white, … WebBJHTERT. Depmap ID: ACH-001018. CCLE Name: BJHTERT_FIBROBLAST. Cell Line Passport: SIDM01927. Cellosaurus RRID: CVCL_6573. Aliases: BJ hTERT.
WebMar 7, 2012 · BjhTERT cells were cultured in IMDM or IMFG supplemented with 10% FBS and PSG. Co-culture assay Tumor cell proliferation on fibroblast monolayers was analyzed in 384-well plates. Fibroblasts were plated in 30–100 μl cell culture medium and cultured for 2–8 days to form confluent monolayers. WebMar 23, 2024 · PR 20-treated cells showed a higher fluorescence intensity of F-actin around the cell cortex than CTRL in both cell lines (U2OS; p = 0.007; 8.4 ± 0.6%, ... Similar to U2OS cells and BJhTERT cells (Figure 3A), PR 20 changed F-actin organization in rat vascular smooth muscle cells (SMCs) ...
WebThe line is sensitive to contact inhibition and shows no signs of transformation. BjhTERT cells were cultured in IMDM or IMFG supplemented with 10% FBS and PSG. Co-culture assay Tumor cell proliferation on fibroblast monolayers was ana-lyzed in 384-well plates. Fibroblasts were plated in 30–100 ll WebCells were stimulated with PDGFBB (Peprotech) for 5 min at 37°C, and the expression of PDGF u0002- receptor and its activation were analyzed by Western blotting using a specific PDGF u0002- receptor antibody (Cell Signaling) and an antibody against phospho-tyrosine (phospho- Y99, sc-7020, Santa Cruz).
WebFeb 27, 2008 · Culture of BJhTERT rho0 and BJhTERT cells Both cell‐types were cultured in a 4 : 1 mixture of Dulbecco’s modified Eagle’s medium and medium 199 supplemented with 10% foetal bovine serum, sodium pyruvate (100 μg/mL), uridine (50 μg/mL) and penicillin/streptomycin (100 IU/mL), in a humidified atmosphere of 5% CO 2 at 37°C.
WebFeb 4, 2024 · We extracted genomic DNA from various cultured human cells infected with MCV (MKL-1, MS-1, WaGa) or without MCV (MCC26, BJhTERT, U2OS). Whereas, MKL … irc 170 f 8WebWe initially infected BJhTert cells (an hTERT-immortalized foreskin fibroblast cell line) with Twist1 to examine changes in cell properties due to Twist1, using a GFP (green … irc 170 b 1 a organizationsWebBJ cells expressing either empty vector, LT.339, or LT.339LFCDK were serum-starved for 48 hours and then harvested for RNA. Survivin mRNA was measured by qRT-PCR and normalized to β-actin mRNA. The experiments were performed three times in duplicate (mean ± SEM, two-tailed t test). Open in viewer Survivin as a target for MCC chemotherapy irc 1802 formWebSep 18, 2014 · The HER2-expressing human ovarian cancer cell line SK-OV-3 ( (HTB-77; ATCC)) was grown in RPMI1640 medium, and the human fibroblast cell line BJhTERT was grown in Minimum Essential Medium (MEM). Both media were supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and 1% penicillin-streptomycin (all reagents from … order boutonniere near meWebCCM collected from BJhTERT whirly + PC3 mRFP coculture (red line) and NCM collected from BJhTERT whirly cells cultured alone (green line) were also tested in 384-well plate … order bowel cancer kitWebSep 21, 2024 · Multiple myeloma (MM) is a B-cell neoplasm characterized by uncontrolled growth of malignant plasma cells and monoclonal immunoglobulins secretion within the bone marrow. It is the second most common hematological malignancy, with a median incidence of 6/100,000 worldwide [ 1 ]. order bowel screening kit scotlandWebCellosaurus BJ1-hTERT (CVCL_6573) Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE). Doubling time: 36 hours (PubMed= 25984343 ). Transfected with: HGNC; 11730; TERT. Derived from sampling site: … irc 183 9 factors